| UPK3A

Uroplakin-3a (UP3a) is a protein that is encoded by the UPK3A gene.UP3a is found in the inner membrane of the urinary bladder and contributes to the strength of that membrane and to the membrane's ability to stretch when the bladder is full. It is also found in the renal pelvis, ureter, and prostatic urethra.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.4	1.77	1.11	0.7	0.45	0.3	0.2	0.07
Corrected OD	2.33	1.7	1.04	0.63	0.38	0.23	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human UPK3A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	95
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	98-108	105

 

| Linearity

Samples were spiked with high concentrations of Human UPK3A and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	94	96
1 : 4	Range (%)	89-103	88-108	105-115
	Average (%)	94	98	108